Français
English
AP-1 (phospho Thr231) rabbit pAb
AO-06-ES5980-100
Antibody Product Overview
| ELK.No | ES5980 |
| Product name | AP-1 (phospho Thr231) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IHC;IF;ELISA |
| Other name | JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3725 |
| Human Swiss-Prot | P05412 |
| Source | Rabbit |
| Isotype | IgG |
| Target | c-JUN |
| Fields | >>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>cAMP signaling pathway;>>Mitophagy - animal;>>Apoptosis;>>Wnt signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Tight junction;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Neurotrophin signaling pathway;>>GnRH signaling pathway;>>Estrogen signaling pathway;>>Oxytocin signaling pathway;>>Relaxin signaling pathway;>>Non-alcoholic fatty liver disease;>>AGE-RAGE signaling pathway in diabetic complications;>>Cocaine addiction;>>Amphetamine addiction;>>Epithelial cell signaling in Helicobacter pylori infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Pertussis;>>Yersinia infection;>>Leishmaniasis;>>Chagas di |
| Gene name | JUN |
| Protein name | Transcription factor AP-1;jun;c-jun；AP-1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16476 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P05627 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24516 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P17325 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human c-Jun around the phosphorylation site of Thr231. AA range:201-250 |
| Specificity | Phospho-AP-1 (T231) Polyclonal Antibody detects endogenous levels of AP-1 protein only when phosphorylated at T231. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 39-42kD |
| Background | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008], |
| Function | function:Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.,PTM:Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Jun subfamily.,similarity:Contains 1 bZIP domain.,subunit:Heterodimer with either FOS or BATF3. Interacts with HIVEP3 (By similarity). Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA., |
| Subcellular location | Nucleus. |
| Expression | Expressed in the developing and adult prostate and prostate cancer cells. |
Additional Images
No image
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using c-Jun (Phospho-Thr231) Antibody
No image
Immunofluorescence analysis of HeLa cells, using c-Jun (Phospho-Thr231) Antibody. The picture on the right is blocked with the phospho peptide.
No image
Immunohistochemistry analysis of paraffin-embedded human testis, using c-Jun (Phospho-Thr231) Antibody. The picture on the right is blocked with the phospho peptide.
No image
Western blot analysis of c-Jun (Phospho-Thr231) Antibody. The lane on the right is blocked with the c-Jun (Phospho-Thr231) peptide.
: AO-06-ES5980-100
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package