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Antibody Product Overview
| ELK.No | ES6025 |
| Product name | ARF6 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | ARF6; ADP-ribosylation factor 6 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 382 |
| Human Swiss-Prot | P62330 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ARF6 |
| Fields | >>Ras signaling pathway;>>Phospholipase D signaling pathway;>>Endocytosis;>>Fc gamma R-mediated phagocytosis;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection |
| Gene name | ARF6 |
| Protein name | ADP-ribosylation factor 6 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11845 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P62331 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 79121 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P62332 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from ARF6 . at AA range: 30-110 |
| Specificity | ARF6 Polyclonal Antibody detects endogenous levels of ARF6 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 20kD |
| Background | ADP ribosylation factor 6(ARF6) Homo sapiens This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008], |
| Function | function:GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.,similarity:Belongs to the small GTPase superfamily.,similarity:Belongs to the small GTPase superfamily. Arf family.,subunit:Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA., |
| Subcellular location | Cytoplasm, cytosol . Cell membrane ; Lipid-anchor . Endosome membrane ; Lipid-anchor . Recycling endosome membrane ; Lipid-anchor . Cell projection, filopodium membrane ; Lipid-anchor . Cell projection, ruffle . Cleavage furrow . Midbody, Midbody ring . Early endosome membrane ; Lipid-anchor . Golgi apparatus, trans-Golgi network membrane ; Lipid-anchor . Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase. Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression. In late stages of cytokinesis, concentrates at the midbody ring/Flemming body (PubMed:23603394). Recruitment to the midbody ring requires both activation by P |
| Expression | Ubiquitous, with higher levels in heart, substantia nigra, and kidney. |
Additional Images
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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES6025-50
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