Français
English
Antibody Product Overview
| ELK.No | ES6103 |
| Product name | Lunatic Fringe rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | LFNG; Beta-1; 3-N-acetylglucosaminyltransferase lunatic fringe; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3955 |
| Human Swiss-Prot | Q8NES3 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Lunatic Fringe |
| Fields | >>Other types of O-glycan biosynthesis;>>Notch signaling pathway;>>Human papillomavirus infection |
| Gene name | LFNG |
| Protein name | Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16848 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O09010 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 170905 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q924T4 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human LFNG. AA range:121-170 |
| Specificity | Lunatic Fringe Polyclonal Antibody detects endogenous levels of Lunatic Fringe protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 42kD |
| Background | This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms |
| Function | alternative products:Experimental confirmation may be lacking for some isoforms,catalytic activity:Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:Defects in LFNG are the cause of spondylocostal dysostosis autosomal recessive type 3 (SCDO3) [MIM:609813]. Autosomal recessive spondylocostal dysostosis is a rare condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the |
| Subcellular location | Golgi apparatus membrane ; Single-pass type II membrane protein . |
| Expression | Kidney, |
Additional Images
No image
Western Blot analysis of 3T3 cells using Lunatic Fringe Polyclonal Antibody diluted at 1:1000
No image
Western blot analysis of lysates from HUVEC and MCF-7 cells, using LFNG Antibody. The lane on the right is blocked with the synthesized peptide.
No image
No image
: AO-06-ES6103-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package