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Antibody Product Overview
| ELK.No | ES6109 |
| Product name | Galectin-8 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | LGALS8; Galectin-8; Gal-8; Po66 carbohydrate-binding protein; Po66-CBP; Prostate carcinoma tumor antigen 1; PCTA-1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3964 |
| Human Swiss-Prot | O00214 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Galectin-8 |
| Fields | |
| Gene name | LGALS8 |
| Protein name | Galectin-8 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 56048 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9JL15 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human LEG8. AA range:61-110 |
| Specificity | Galectin-8 Polyclonal Antibody detects endogenous levels of Galectin-8 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 36kD |
| Background | This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008], |
| Function | domain:Contains two homologous but distinct carbohydrate-binding domains.,online information:Galectin-8 C-terminal CRD,online information:Galectin-8 long isoform,similarity:Contains 2 galectin domains.,tissue specificity:Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy., |
| Subcellular location | Cytoplasmic vesicle . Cytoplasm, cytosol . |
| Expression | Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy. |
Additional Images
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Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
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Western Blot analysis of 3T3 cells using Galectin-8 Polyclonal Antibody diluted at 1:1000
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Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using LEG8 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from NIH/3T3 cells, using LEG8 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES6109-100
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