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Smad2 (phospho Ser250) rabbit pAb
AO-06-ES6154-50
Antibody Product Overview
| ELK.No | ES6154 |
| Product name | Smad2 (phospho Ser250) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4087 |
| Human Swiss-Prot | Q15796 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Smad2 |
| Fields | >>Cell cycle;>>Endocytosis;>>Cellular senescence;>>TGF-beta signaling pathway;>>Apelin signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Th17 cell differentiation;>>Relaxin signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Chagas disease;>>Human T-cell leukemia virus 1 infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Colorectal cancer;>>Pancreatic cancer;>>Hepatocellular carcinoma;>>Gastric cancer;>>Inflammatory bowel disease;>>Diabetic cardiomyopathy |
| Gene name | SMAD2 |
| Protein name | Mothers against decapentaplegic homolog 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17126 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q62432 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 29357 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O70436 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Ser250. AA range:216-265 |
| Specificity | Phospho-Smad2 (S250) Polyclonal Antibody detects endogenous levels of Smad2 protein only when phosphorylated at S250. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 65kD |
| Background | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation |
| Function | disease:Defects in SMAD2 are found in sporadic cases of colorectal carcinoma.,function:Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.,PTM:Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo.,PTM:In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.,PTM:Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degr |
| Subcellular location | Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity). . |
| Expression | Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad2 (Phospho-Ser250) Antibody
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Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using Smad2 (Phospho-Ser250) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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