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FoxO4 (phospho Thr451) rabbit pAb
AO-06-ES6257-100
Antibody Product Overview
| ELK.No | ES6257 |
| Product name | FoxO4 (phospho Thr451) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | FOXO4; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 4303 |
| Human Swiss-Prot | P98177 |
| Source | Rabbit |
| Isotype | IgG |
| Target | FoxO4 |
| Fields | >>Ras signaling pathway;>>FoxO signaling pathway;>>Shigellosis |
| Gene name | FOXO4 |
| Protein name | Forkhead box protein O4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 54601 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9WVH3 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FOXO4 around the phosphorylation site of Thr451. AA range:417-466 |
| Specificity | Phospho-FoxO4 (T451) Polyclonal Antibody detects endogenous levels of FoxO4 protein only when phosphorylated at T451. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 55kD |
| Background | This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010], |
| Function | disease:A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.,function:Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.,pharmaceutical:A constitutively active FOXO4 mutant where phosphorylation sites Thr-32, Ser-187 and Ser-262 have been mutated to alanine may have therapeutic potential in ERBB2/HER2-overexpressing cancers as it inhibits ERBB2-mediated cell survival, transformation and tumorigenicity.,PTM:Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Dea |
| Subcellular location | Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm. |
| Expression | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FOXO4 (Phospho-Thr451) Antibody
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Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 5', using FOXO4 (Phospho-Thr451) Antibody. The lane on the right is blocked with the phospho peptide.
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: AO-06-ES6257-100
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