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Trk B (phospho Tyr516) rabbit pAb
AO-06-ES6429-100
Antibody Product Overview
| ELK.No | ES6429 |
| Product name | Trk B (phospho Tyr516) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | NTRK2; TRKB; BDNF/NT-3 growth factors receptor; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 4915 |
| Human Swiss-Prot | Q16620 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Trk B |
| Fields | >>MAPK signaling pathway;>>Ras signaling pathway;>>Calcium signaling pathway;>>PI3K-Akt signaling pathway;>>Neurotrophin signaling pathway;>>Alcoholism |
| Gene name | NTRK2 |
| Protein name | BDNF/NT-3 growth factors receptor |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18212 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P15209 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25054 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q63604 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Trk B around the phosphorylation site of Tyr515. AA range:481-530 |
| Specificity | Phospho-Trk B (Y516) Polyclonal Antibody detects endogenous levels of Trk B protein only when phosphorylated at Y516. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 145kD |
| Background | This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014], |
| Function | alternative products:Additional isoforms seem to exist,catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.,PTM:Ligand-mediated auto-phosphorylation.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,similarity:Contains 2 LRR (leucine-rich) repeats.,subunit:Exists in a dynamic equ |
| Subcellular location | Cell membrane ; Single-pass type I membrane protein . Endosome membrane ; Single-pass type I membrane protein . Early endosome membrane . Cell projection, axon . Cell projection, dendrite . Cytoplasm, perinuclear region . Cell junction, synapse, postsynaptic density . Internalized to endosomes upon ligand-binding. . |
| Expression | Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is mainly expressed in the brain but also detected in other tissues including pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in the brain. |
Additional Images
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Western Blot analysis of RAT-MUSCLE cells using Phospho-Trk B (Y516) Polyclonal Antibody diluted at 1:1000
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Trk B (Phospho-Tyr515) Antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using Trk B (Phospho-Tyr515) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES6429-100
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