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Antibody Product Overview
| ELK.No | ES6439 |
| Product name | Wee 2 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | WEE2; WEE1B; Wee1-like protein kinase 2; Wee1-like protein kinase 1B; Wee1B kinase |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 494551 |
| Human Swiss-Prot | P0C1S8 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Wee 2 |
| Fields | >>Cell cycle;>>Human immunodeficiency virus 1 infection |
| Gene name | WEE2 |
| Protein name | Wee1-like protein kinase 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q66JT0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human WEE2. AA range:151-200 |
| Specificity | Wee 2 Polyclonal Antibody detects endogenous levels of Wee 2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 60kD |
| Background | catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Phosphorylates and inhibits CDC2. May act as a negative regulator of entry into mitosis (G2 to M transition).,PTM:Phosphorylated .,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed in testis., |
| Function | catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Phosphorylates and inhibits CDC2. May act as a negative regulator of entry into mitosis (G2 to M transition).,PTM:Phosphorylated .,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed in testis., |
| Subcellular location | Nucleus . |
| Expression | Expressed in oocytes (at protein level) (PubMed:29606300). May also be expressed in testis (PubMed:11029659). |
Additional Images
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Western blot analysis of lysates from HUVEC cells, using WEE2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from K562 cells using WEE2 antibody.
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: AO-06-ES6439-100
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