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Antibody Product Overview
| ELK.No | ES6516 |
| Product name | CYP39A1 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | CYP39A1; 24-hydroxycholesterol 7-alpha-hydroxylase; Cytochrome P450 39A1; hCYP39A1; Oxysterol 7-alpha-hydroxylase |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 51302 |
| Human Swiss-Prot | Q9NYL5 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CYP39A1 |
| Fields | >>Primary bile acid biosynthesis |
| Gene name | CYP39A1 |
| Protein name | 24-hydroxycholesterol 7-alpha-hydroxylase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 56050 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9JKJ9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cytochrome P450 39A1. AA range:361-410 |
| Specificity | CYP39A1 Polyclonal Antibody detects endogenous levels of CYP39A1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 54kD |
| Background | cytochrome P450 family 39 subfamily A member 1(CYP39A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is involved in the conversion of cholesterol to bile acids. Its substrates include the oxysterols 25-hydroxycholesterol, 27-hydroxycholesterol and 24-hydroxycholesterol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013], |
| Function | catalytic activity:(24R)-cholest-5-ene-3-beta,24-diol + NADPH + O(2) = (24R)-cholest-5-ene-3-beta,7-alpha,24-triol + NADP(+) + H(2)O.,cofactor:Heme group.,function:Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha-hydroxylated product.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Liver specific., |
| Subcellular location | Endoplasmic reticulum membrane ; Multi-pass membrane protein . Microsome membrane ; Multi-pass membrane protein . |
| Expression | Liver specific. |
Additional Images
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Immunofluorescence analysis of A549 cells, using Cytochrome P450 39A1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO and HUVEC cells, using Cytochrome P450 39A1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES6516-50
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