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Antibody Product Overview
| ELK.No | ES6548 |
| Product name | ERGIC-3 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | ERGIC3; C20orf47; ERV46; SDBCAG84; CGI-54; Endoplasmic reticulum-Golgi intermediate compartment protein 3; Serologically defined breast cancer antigen NY-BR-84 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 51614 |
| Human Swiss-Prot | Q9Y282 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ERGIC-3 |
| Fields | |
| Gene name | ERGIC3 |
| Protein name | Endoplasmic reticulum-Golgi intermediate compartment protein 3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 66366 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9CQE7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ERGI3. AA range:321-370 |
| Specificity | ERGIC-3 Polyclonal Antibody detects endogenous levels of ERGIC-3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 43kD |
| Background | function:Possible role in transport between endoplasmic reticulum and Golgi.,similarity:Belongs to the ERGIC family.,subcellular location:Cycles between the endoplasmic reticulum and the Golgi.,subunit:Interacts with ERGIC1/ERGIC32., |
| Function | function:Possible role in transport between endoplasmic reticulum and Golgi.,similarity:Belongs to the ERGIC family.,subcellular location:Cycles between the endoplasmic reticulum and the Golgi.,subunit:Interacts with ERGIC1/ERGIC32., |
| Subcellular location | Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein . Golgi apparatus, cis-Golgi network membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cycles between the endoplasmic reticulum and the Golgi. |
| Expression | Brain,Embryo,Fetal liver,Liver,Mammary gland,Skin, |
Additional Images
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Immunofluorescence analysis of HepG2 cells, using ERGI3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO and NIH/3T3 cells, using ERGI3 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES6548-100
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