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cPLA2 (phospho Ser505) rabbit pAb
AO-06-ES6599-50
Antibody Product Overview
| ELK.No | ES6599 |
| Product name | cPLA2 (phospho Ser505) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5321 |
| Human Swiss-Prot | P47712 |
| Source | Rabbit |
| Isotype | IgG |
| Target | cPLA2 |
| Fields | >>Glycerophospholipid metabolism;>>Ether lipid metabolism;>>Arachidonic acid metabolism;>>Linoleic acid metabolism;>>alpha-Linolenic acid metabolism;>>Metabolic pathways;>>MAPK signaling pathway;>>Ras signaling pathway;>>Phospholipase D signaling pathway;>>Necroptosis;>>Vascular smooth muscle contraction;>>VEGF signaling pathway;>>Platelet activation;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Glutamatergic synapse;>>Serotonergic synapse;>>Long-term depression;>>Inflammatory mediator regulation of TRP channels;>>GnRH signaling pathway;>>Ovarian steroidogenesis;>>Oxytocin signaling pathway;>>Choline metabolism in cancer |
| Gene name | PLA2G4A |
| Protein name | Cytosolic phospholipase A2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18783 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P47713 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | P50393 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human c-PLA2 around the phosphorylation site of Ser505. AA range:471-520 |
| Specificity | Phospho-cPLA2 (S505) Polyclonal Antibody detects endogenous levels of cPLA2 protein only when phosphorylated at S505. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 110kD |
| Background | This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015], |
| Function | catalytic activity:2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate.,catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.,domain:The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).,enzyme regulation:Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca(2+).,function:Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.,PTM:Activated by phosphorylation at both Ser-505 and Ser-727.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PLA2c domain.,subcellular location:Translocates to membrane ve |
| Subcellular location | Cytoplasm . Golgi apparatus membrane . Nucleus envelope. Translocates to intracellular membranes in a calcium-dependent way. . |
| Expression | Expressed in various cells and tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level) (PubMed:25102815). |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,cPLA2 (phospho Ser505) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
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Western blot analysis of VEC using p-cPLA2 (S505) antibody. Antibody was diluted at 1:1000
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using c-PLA2 (Phospho-Ser505) Antibody
: AO-06-ES6599-50
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