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Histone H2B (Acetyl Lys15) rabbit pAb
AO-06-ES6640-50
Antibody Product Overview
| ELK.No | ES6640 |
| Product name | Histone H2B (Acetyl Lys15) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s;H2BK15AC |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 54145 |
| Human Swiss-Prot | P57053 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Histone H2B |
| Fields | >>Neutrophil extracellular trap formation;>>Alcoholism;>>Viral carcinogenesis;>>Systemic lupus erythematosus |
| Gene name | H2BFS |
| Protein name | Histone H2B type F-S |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys15. AA range:1-50 |
| Specificity | Acetyl-Histone H2B (K15) Polyclonal Antibody detects endogenous levels of Histone H2B protein only when acetylated at K15. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 20kD |
| Background | H2BFS (H2B Histone Family Member S (Pseudogene)) is a Pseudogene. Diseases associated with H2BFS include endometrial stromal sarcoma. Among its related pathways are Packaging Of Telomere Ends. GO annotations related to this gene include sequence-specific DNA binding and protein heterodimerization activity. An important paralog of this gene is HIST1H2BH. |
| Function | DNA packaging, chromatin organization, chromatin assembly or disassembly, nucleosome assembly, defense response, response to bacterium, chromatin assembly, cellular macromolecular complex subunit organization, cellular macromolecular complex assembly, nucleosome organization, defense response to bacterium, macromolecular complex subunit organization, chromosome organization, macromolecular complex assembly, protein-DNA complex assembly, |
| Subcellular location | Nucleus. Chromosome. |
| Expression |
Additional Images
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Western Blot analysis of 3T3 cells using Acetyl-Histone H2B (K15) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys15) Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES6640-50
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