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TRAP220 (phospho Thr1457) rabbit pAb
AO-06-ES6670-50
Antibody Product Overview
| ELK.No | ES6670 |
| Product name | TRAP220 (phospho Thr1457) rabbit pAb |
| Reactivity | Human;Mouse;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | MED1; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2; Mediator of RNA polymerase II transcription subunit 1; Activator-recruited cofactor 205 kDa component; ARC205; Mediator complex subunit 1; Peroxiso |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5469 |
| Human Swiss-Prot | Q15648 |
| Source | Rabbit |
| Isotype | IgG |
| Target | TRAP220 |
| Fields | >>Endocrine resistance;>>Thyroid hormone signaling pathway |
| Gene name | MED1 |
| Protein name | Mediator of RNA polymerase II transcription subunit 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 19014 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q925J9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PPAR-BP around the phosphorylation site of Thr1457. AA range:1423-1472 |
| Specificity | Phospho-TRAP220 (T1457) Polyclonal Antibody detects endogenous levels of TRAP220 protein only when phosphorylated at T1457. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 168kD |
| Background | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008], |
| Function | function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.,PTM:Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the Mediator complex subunit 1 family.,subcellular location:A subset of the protein may enter the nucleol |
| Subcellular location | Nucleus . A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3. |
| Expression | Ubiquitously expressed. |
Additional Images
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Immunofluorescence analysis of HeLa cells, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human heart, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using PPAR-BP (Phospho-Thr1457) Antibody. The lane on the right is blocked with the phospho peptide.
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: AO-06-ES6670-50
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