Français
English
Antibody Product Overview
| ELK.No | ES6675 |
| Product name | Arkadia rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | RNF111; E3 ubiquitin-protein ligase Arkadia; RING finger protein 111 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 54778 |
| Human Swiss-Prot | Q6ZNA4 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Arkadia |
| Fields | |
| Gene name | RNF111 |
| Protein name | E3 ubiquitin-protein ligase Arkadia |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 93836 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q99ML9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RNF111. AA range:901-950 |
| Specificity | Arkadia Polyclonal Antibody detects endogenous levels of Arkadia protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 110kD |
| Background | ring finger protein 111(RNF111) Homo sapiens The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012], |
| Function | function:Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Upon TGF-beta treatment, translocates from nucleus to cytosol.,subunit:Interacts with SMAD6, SMAD7, AXIN1, AXIN2 and SKIL isoform SNON. Part of a complex containing RNF111, AXIN1 and SMAD7.,tissue specificity:Broadly expressed., |
| Subcellular location | Nucleus . Cytoplasm . Nucleus, PML body . Upon TGF-beta treatment, translocates from nucleus to cytosol. . |
| Expression | Broadly expressed. |
Additional Images
No image
Western blot analysis of RNF111 Antibody. The lane on the right is blocked with the RNF111 peptide.
No image
Western blot analysis of the lysates from Jurkat cells using RNF111 antibody.
No image
No image
: AO-06-ES6675-100
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package