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Antibody Product Overview
| ELK.No | ES6682 |
| Product name | CCDC99 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | CCDC99; Protein Spindly; hSpindly; Arsenite-related gene 1 protein; Coiled-coil domain-containing protein 99; Rhabdomyosarcoma antigen MU-RMS-40.4A |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 54908 |
| Human Swiss-Prot | Q96EA4 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CCDC99 |
| Fields | |
| Gene name | CCDC99 |
| Protein name | Protein Spindly |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q923A2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CCDC99. AA range:541-590 |
| Specificity | CCDC99 Polyclonal Antibody detects endogenous levels of CCDC99 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 70kD |
| Background | This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. [provided by RefSeq, Jul 2016], |
| Function | mitotic sister chromatid segregation, M phase of mitotic cell cycle, establishment of mitotic spindle orientation,microtubule cytoskeleton organization, mitotic cell cycle, M phase, nuclear division, sister chromatid segregation, cell morphogenesis, cytoskeleton organization, microtubule-based process, cell cycle, chromosome segregation, mitosis,mitotic metaphase plate congression, establishment or maintenance of cell polarity, protein localization, cell cycle process, cell cycle phase, establishment of cell polarity, cellular component morphogenesis, protein localization to kinetochore, cellular protein localization, establishment of mitotic spindle localization, organelle fission, chromosome localization, chromosome organization, establishment of spindle localization, establishment of spindle orientation,establishment of chromosome localization, metaphase plate congression, organelle l |
| Subcellular location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex. |
| Expression |
Additional Images
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Western Blot analysis of 3T3 cells using CCDC99 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of CCDC99 Antibody. The lane on the right is blocked with the CCDC99 peptide.
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Western blot analysis of the lysates from K562 cells using CCDC99 antibody.
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: AO-06-ES6682-50
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