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Antibody Product Overview
| ELK.No | ES6745 |
| Product name | Pki α rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IHC;IF;ELISA |
| Other name | PKIA; PRKACN1; cAMP-dependent protein kinase inhibitor alpha; PKI-alpha; cAMP-dependent protein kinase inhibitor; muscle/brain isoform |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5569 |
| Human Swiss-Prot | P61925 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Pki α |
| Fields | >>Alcoholism |
| Gene name | PKIA |
| Protein name | cAMP-dependent protein kinase inhibitor alpha |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18767 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P63248 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 114906 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P63249 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IPKA. AA range:10-59 |
| Specificity | Pki α Polyclonal Antibody detects endogenous levels of Pki α protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 8kD |
| Observed band (KD) | |
| Background | The protein encoded by this gene is a member of the cAMP-dependent protein kinase (PKA) inhibitor family. This protein was demonstrated to interact with and inhibit the activities of both C alpha and C beta catalytic subunits of the PKA. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008], |
| Function | function:Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.,miscellaneous:The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.,similarity:Belongs to the PKI family., |
| Subcellular location | nucleus,cytoplasm, |
| Expression | Skeletal muscle, |
Additional Images
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Immunofluorescence analysis of HeLa cells, using IPKA Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IPKA Antibody. The picture on the right is blocked with the synthesized peptide.
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: AO-06-ES6745-100
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