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DNA-PKCS (phospho Thr2638) rabbit pAb
AO-06-ES6804-50
Antibody Product Overview
| ELK.No | ES6804 |
| Product name | DNA-PKCS (phospho Thr2638) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | IF;ELISA |
| Other name | PRKDC; HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5591 |
| Human Swiss-Prot | P78527 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DNA-PKCS |
| Fields | >>Non-homologous end-joining;>>Cell cycle |
| Gene name | PRKDC |
| Protein name | DNA-dependent protein kinase catalytic subunit |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 19090 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P97313 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DNA-PK around the phosphorylation site of Thr2638. AA range:2604-2653 |
| Specificity | Phospho-DNA-PKCS (T2638) Polyclonal Antibody detects endogenous levels of DNA-PKCS protein only when phosphorylated at T2638. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 469kD |
| Observed band (KD) | |
| Background | This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Inhibited by wortmannin. Activity of the enzyme seems to be attenuated by autophosphorylation.,function:Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of |
| Subcellular location | Nucleus . Nucleus, nucleolus . |
| Expression | Brain,Cervix carcinoma,Epithelium,Fetal lung,Placen |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DNA-PK (Phospho-Thr2638) Antibody
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Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using DNA-PK (Phospho-Thr2638) Antibody. The picture on the right is blocked with the phospho peptide.
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: AO-06-ES6804-50
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