Français
English
Antibody Product Overview
| ELK.No | ES6817 |
| Product name | ERK 3 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | MAPK6; ERK3; PRKM6; Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5597 |
| Human Swiss-Prot | Q16659 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ERK 3 |
| Fields | >>IL-17 signaling pathway |
| Gene name | MAPK6 |
| Protein name | Mitogen-activated protein kinase 6 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 50772 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61532 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 58840 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P27704 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p97 MAPK. AA range:461-510 |
| Specificity | ERK 3 Polyclonal Antibody detects endogenous levels of ERK 3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 85kD |
| Background | The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation.,function:Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.,PTM:Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts., |
| Subcellular location | Cytoplasm . Nucleus . Translocates to the cytoplasm following interaction with MAPKAPK5. . |
| Expression | Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts. |
Additional Images
No image
Western blot analysis of Hela Lysate, antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
No image
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
No image
Immunofluorescence analysis of MCF7 cells, using p97 MAPK Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Western blot analysis of lysates from K562 cells, using p97 MAPK Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES6817-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package