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MEK-7 (phospho Ser271) rabbit pAb
AO-06-ES6833-100
Antibody Product Overview
| ELK.No | ES6833 |
| Product name | MEK-7 (phospho Ser271) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | MAP2K7; JNKK2; MEK7; MKK7; PRKMK7; SKK4; Dual specificity mitogen-activated protein kinase kinase 7; MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; Stress-activated protein kinase kinase 4; SAPK kinase 4; S |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5609 |
| Human Swiss-Prot | O14733 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MKK7 |
| Fields | >>MAPK signaling pathway;>>ErbB signaling pathway;>>Protein processing in endoplasmic reticulum;>>Osteoclast differentiation;>>Tight junction;>>Toll-like receptor signaling pathway;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>TNF signaling pathway;>>Neurotrophin signaling pathway;>>GnRH signaling pathway;>>Relaxin signaling pathway;>>Alcoholic liver disease;>>Alzheimer disease;>>Huntington disease;>>Pathways of neurodegeneration - multiple diseases;>>Salmonella infection;>>Yersinia infection;>>Hepatitis B;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Chemical carcinogenesis - reactive oxygen species;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis |
| Gene name | MAP2K7 |
| Protein name | Dual specificity mitogen-activated protein kinase kinase 7 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 26400 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8CE90 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 363855 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q4KSH7 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MAP2K7 around the phosphorylation site of Ser271. AA range:236-285 |
| Specificity | Phospho-MEK-7 (S271) Polyclonal Antibody detects endogenous levels of MEK-7 protein only when phosphorylated at S271. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 47kD |
| Background | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation by specific MAP kinase kinase kinases such as MAP3K1/MEKK1, MAP3K3/MEKK3, MAP3K11/MLK3 and MAP3K12/DLK.,function:Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.,PTM:Activated by phosphorylation on Ser/Thr.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle., |
| Subcellular location | Nucleus. Cytoplasm . |
| Expression | Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. |
Additional Images
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Western Blot analysis of 3T3 cells using Phospho-MEK-7 (S271) Polyclonal Antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP2K7 (Phospho-Ser271) Antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K7 (Phospho-Ser271) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from NIH/3T3 cells treated with insulin 0.01U/ml 15' and NIH/3T3 cells treated with EGF 200ng/ml 30', using MAP2K7 (Phospho-Ser271) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES6833-100
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