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Antibody Product Overview
| ELK.No | ES7043 |
| Product name | RGS12 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | RGS12; Regulator of G-protein signaling 12; RGS12 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 6002 |
| Human Swiss-Prot | O14924 |
| Source | Rabbit |
| Isotype | IgG |
| Target | RGS12 |
| Fields | |
| Gene name | RGS12 |
| Protein name | Regulator of G-protein signaling 12 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 71729 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8CGE9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 54292 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O08774 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RGS12. AA range:141-190 |
| Specificity | RGS12 Polyclonal Antibody detects endogenous levels of RGS12 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 160kD |
| Background | This gene encodes a member of the 'regulator of G protein signaling' (RGS) gene family. The encoded protein may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined. [provided by RefSeq, Jul 2008], |
| Function | function:Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 GoLoco domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PID domain.,similarity:Contains 1 RGS domain.,similarity:Contains 2 RBD (Ras-binding) domains.,tissue specificity:Isoform 3 is brain specific., |
| Subcellular location | Nucleus . Cytoplasm . Cell projection, dendrite . Cell junction, synapse .; [Isoform 5]: Nucleus matrix . Also localized to discrete nuclear foci that are distinct from sites of RNA processing, PML nuclear bodies, and PcG domains. . |
| Expression | Isoform 3 is brain specific. |
Additional Images
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Western Blot analysis of 3T3 cells using RGS12 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of RGS12 Antibody. The lane on the right is blocked with the RGS12 peptide.
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Western blot analysis of the lysates from HUVECcells using RGS12 antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES7043-50
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