Français
English
Antibody Product Overview
| ELK.No | ES7043 |
| Product name | RGS12 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | RGS12; Regulator of G-protein signaling 12; RGS12 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 6002 |
| Human Swiss-Prot | O14924 |
| Source | Rabbit |
| Isotype | IgG |
| Target | RGS12 |
| Fields | |
| Gene name | RGS12 |
| Protein name | Regulator of G-protein signaling 12 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 71729 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8CGE9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 54292 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O08774 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RGS12. AA range:141-190 |
| Specificity | RGS12 Polyclonal Antibody detects endogenous levels of RGS12 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 160kD |
| Background | This gene encodes a member of the 'regulator of G protein signaling' (RGS) gene family. The encoded protein may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined. [provided by RefSeq, Jul 2008], |
| Function | function:Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 GoLoco domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PID domain.,similarity:Contains 1 RGS domain.,similarity:Contains 2 RBD (Ras-binding) domains.,tissue specificity:Isoform 3 is brain specific., |
| Subcellular location | Nucleus . Cytoplasm . Cell projection, dendrite . Cell junction, synapse .; [Isoform 5]: Nucleus matrix . Also localized to discrete nuclear foci that are distinct from sites of RNA processing, PML nuclear bodies, and PcG domains. . |
| Expression | Isoform 3 is brain specific. |
Additional Images
No image
Western Blot analysis of 3T3 cells using RGS12 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
No image
Western blot analysis of RGS12 Antibody. The lane on the right is blocked with the RGS12 peptide.
No image
Western blot analysis of the lysates from HUVECcells using RGS12 antibody.
No image
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES7043-100
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package