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Rsk-1 (phospho Ser380) rabbit pAb
AO-06-ES7080-100
Antibody Product Overview
| ELK.No | ES7080 |
| Product name | Rsk-1 (phospho Ser380) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | RPS6KA1; MAPKAPK1A; RSK1; Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAP |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 6195 |
| Human Swiss-Prot | Q15418 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rsk-1 |
| Fields | >>MAPK signaling pathway;>>Oocyte meiosis;>>mTOR signaling pathway;>>Thermogenesis;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Progesterone-mediated oocyte maturation;>>Insulin resistance;>>Yersinia infection;>>Chemical carcinogenesis - receptor activation |
| Gene name | RPS6KA1 |
| Protein name | Ribosomal protein S6 kinase alpha-1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P18653 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 81771 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q63531 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p90 RSK around the phosphorylation site of Ser380. AA range:346-395 |
| Specificity | Phospho-Rsk-1 (S380) Polyclonal Antibody detects endogenous levels of Rsk-1 protein only when phosphorylated at S380. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 83kD |
| Background | ribosomal protein S6 kinase A1(RPS6KA1) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,cofactor:Magnesium.,enzyme regulation:Activated by multiple phosphorylations on threonine and serine residues.,function:Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.,PTM:Autophosphorylated on Ser-380, as part of the activation process.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic s |
| Subcellular location | Nucleus. Cytoplasm. |
| Expression | Colon,Epithelium, |
Additional Images
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Western Blot analysis of 293 cells using Phospho-Rsk-1 (S380) Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p90 RSK (Phospho-Ser380) Antibody
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Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using p90 RSK (Phospho-Ser380) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES7080-100
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