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Antibody Product Overview
| ELK.No | ES7152 |
| Product name | ACOT1 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | ACOT1; CTE1; Acyl-coenzyme A thioesterase 1; Acyl-CoA thioesterase 1; CTE-I; CTE-Ib; Inducible cytosolic acyl-coenzyme A thioester hydrolase; Long chain acyl-CoA thioester hydrolase; Long chain acyl-CoA hydrolase |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 641371 |
| Human Swiss-Prot | Q86TX2 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ACOT1 |
| Fields | >>Fatty acid elongation;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Ovarian steroidogenesis |
| Gene name | ACOT1 |
| Protein name | Acyl-coenzyme A thioesterase 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | O55137 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACOT1. AA range:91-140 |
| Specificity | ACOT1 Polyclonal Antibody detects endogenous levels of ACOT1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 46kD |
| Background | catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer., |
| Function | catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer., |
| Subcellular location | Cytoplasm, cytosol . |
| Expression | Neuroblastoma, |
Additional Images
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of MCF7 cells, using ACOT1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using ACOT1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HT-29 cells using ACOT1 antibody.
: AO-06-ES7152-100
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