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Antibody Product Overview
| ELK.No | ES7167 |
| Product name | GalNAc4ST-1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | CHST8; Carbohydrate sulfotransferase 8; GalNAc-4-O-sulfotransferase 1; GalNAc-4-ST1; GalNAc4ST-1; N-acetylgalactosamine-4-O-sulfotransferase 1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 64377 |
| Human Swiss-Prot | Q9H2A9 |
| Source | Rabbit |
| Isotype | IgG |
| Target | GalNAc4ST-1 |
| Fields | >>Various types of N-glycan biosynthesis;>>Metabolic pathways |
| Gene name | CHST8 |
| Protein name | Carbohydrate sulfotransferase 8 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 68947 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8BQ86 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CHST8. AA range:341-390 |
| Specificity | GalNAc4ST-1 Polyclonal Antibody detects endogenous levels of GalNAc4ST-1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | The protein encoded by this gene belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011], |
| Function | caution:PubMed:10988300 reports the possible existence of a secreted isoform starting at Met-119. However, they do not provide any experimental evidence.,function:Catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Only active against terminal GalNAcbeta1,GalNAcbeta. Not active toward chondroitin.,induction:Down-regulated (17-fold) in prion-infected cells.,similarity:Belongs to the sulfotransferase 2 family.,tissue specificity:Predominantly expressed in pituitary gland. In brain, it is expressed in pituitary gland, cerebellum, medulla oblongata, pons, thalamus and spinal cord. Expressed at lower level in lung, spleen, adrenal gland, placenta, prostate, testis, mammary glan |
| Subcellular location | Golgi apparatus membrane ; Single-pass type II membrane protein . |
| Expression | Predominantly expressed in pituitary gland. In brain, it is expressed in pituitary gland, cerebellum, medulla oblongata, pons, thalamus and spinal cord. Expressed in the epidermis. Expressed at lower level in lung, spleen, adrenal gland, placenta, prostate, testis, mammary gland and trachea. |
Additional Images
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Western blot analysis of lysates from K562 cells, using CHST8 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HT-29 cells using CHST8 antibody.
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: AO-06-ES7167-50
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