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Antibody Product Overview
| ELK.No | ES7184 |
| Product name | Endophilin I rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | SH3GL2; CNSA2; SH3D2A; Endophilin-A1; EEN-B1; Endophilin-1; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 6456 |
| Human Swiss-Prot | Q99962 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Endophilin I |
| Fields | >>Endocytosis |
| Gene name | SH3GL2 |
| Protein name | Endophilin-A1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 20404 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q62420 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 116743 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O35179 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from Endophilin I . at AA range: 30-110 |
| Specificity | Endophilin I Polyclonal Antibody detects endogenous levels of Endophilin I protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 39kD |
| Background | domain:An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.,function:Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.,miscellaneous:HeLa cells expressing the N-BAR domain of SH3GL2 show tubulation of the plasma membrane. The N-BAR domain binds liposomes and induces formation of tubules from liposomes. The N-terminal amphipathic helix is required for liposome binding. The second amphipathic helix enhances liposome tubulation.,similarity:Belongs to the endophilin family.,similarity:Contains 1 BAR domain.,similarity:Contains 1 SH3 domain.,subcellular location:Concentrated in presynaptic nerve terminals in neurons.,subunit:Monomer; in cytoplasm. Homodimer; when associated with membranes (By similarity). Interacts with SYNJ1 and DNM1. Interacts with MAP4K3; the interaction appears to regulate MAP4K3-mediated JNK activation. Interacts with PDCD6IP.,tissue specificity:Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum., |
| Function | domain:An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.,function:Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.,miscellaneous:HeLa cells expressing the N-BAR domain of SH3GL2 show tubulation of the plasma membrane. The N-BAR domain binds liposomes and induces formation of tubules from liposomes. The N-terminal amphipathic helix is required for liposome binding. The second amphipathic helix enhances liposome tubulation.,similarity:Belongs to the endophilin family.,similarity:Contains 1 BAR domain.,similarity:Contains 1 SH3 domain.,subcel |
| Subcellular location | Cytoplasm . Membrane ; Peripheral membrane protein . Early endosome . Cell junction, synapse, presynapse . |
| Expression | Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum. |
Additional Images
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Western blot analysis of SH3GL2 Antibody. The lane on the right is blocked with the SH3GL2 peptide.
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: AO-06-ES7184-50
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