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Antibody Product Overview
| ELK.No | ES7210 |
| Product name | MRP-L32 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | MRPL32; HSPC283; 39S ribosomal protein L32; mitochondrial; L32mt; MRP-L32 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 64983 |
| Human Swiss-Prot | Q9BYC8 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MRP-L32 |
| Fields | >>Ribosome |
| Gene name | MRPL32 |
| Protein name | 39S ribosomal protein L32 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9DCI9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPL32. AA range:101-150 |
| Specificity | MRP-L32 Polyclonal Antibody detects endogenous levels of MRP-L32 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 21kD |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008], |
| Function | similarity:Belongs to the ribosomal protein L32P family., |
| Subcellular location | Mitochondrion . |
| Expression | Lymph,Trachea,Umbilical cord blood, |
Additional Images
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of A549 cells, using MRPL32 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from MCF-7, HepG2, and Jurkat cells, using MRPL32 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using MRPL32 antibody.
: AO-06-ES7210-50
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