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Sp1 (phospho Thr453) rabbit pAb
AO-06-ES7256-100
Antibody Product Overview
| ELK.No | ES7256 |
| Product name | Sp1 (phospho Thr453) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | SP1; TSFP1; Transcription factor Sp1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 6667 |
| Human Swiss-Prot | P08047 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Sp1 |
| Fields | >>Endocrine resistance;>>Mitophagy - animal;>>TGF-beta signaling pathway;>>Estrogen signaling pathway;>>Cortisol synthesis and secretion;>>Parathyroid hormone synthesis, secretion and action;>>Cushing syndrome;>>Huntington disease;>>Spinocerebellar ataxia;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Breast cancer;>>Choline metabolism in cancer;>>Diabetic cardiomyopathy |
| Gene name | SP1 |
| Protein name | Transcription factor Sp1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 20683 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O89090 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24790 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q01714 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr453. AA range:421-470 |
| Specificity | Phospho-Sp1 (T453) Polyclonal Antibody detects endogenous levels of Sp1 protein only when phosphorylated at T453. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 90kD |
| Background | The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014], |
| Function | function:Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.,PTM:O-glycosylated; contains N-acetylglucosamine side chains.,similarity:Belongs to the Sp1 C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr. Interacts with SV40 VP2/3 proteins. Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between the two proteins in DNA binding., |
| Subcellular location | Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location. |
| Expression | Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels. |
Additional Images
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Immunofluorescence analysis of HeLa cells, using SP1 (Phospho-Thr453) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using SP1 (Phospho-Thr453) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from A549 cells, using SP1 (Phospho-Thr453) Antibody. The lane on the right is blocked with the phospho peptide.
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: AO-06-ES7256-100
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