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SSB (phospho Ser366) rabbit pAb
AO-06-ES7288-100
Antibody Product Overview
| ELK.No | ES7288 |
| Product name | SSB (phospho Ser366) rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | SSB; Lupus La protein; La autoantigen; La ribonucleoprotein; Sjoegren syndrome type B antigen; SS-B |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 6741 |
| Human Swiss-Prot | P05455 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SSB |
| Fields | >>Systemic lupus erythematosus |
| Gene name | SSB |
| Protein name | Lupus La protein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P32067 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SSB around the phosphorylation site of Ser366. AA range:341-390 |
| Specificity | Phospho-SSB (S366) Polyclonal Antibody detects endogenous levels of SSB protein only when phosphorylated at S366. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 47kD |
| Background | Sjogren syndrome antigen B(SSB) Homo sapiens The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014], |
| Function | disease:Sera from patients with systemic lupus erythematosus (SLE) often contain antibodies that react with the normal cellular La protein as if this antigen was foreign.,function:La protein plays a role in the transcription of RNA polymerase III. It is most probably a transcription termination factor. Binds to the 3' termini of virtually all nascent polymerase III transcripts. It is associated with precursor forms of RNA polymerase III transcripts including tRNA and 4.5S, 5S, 7S, and 7-2 RNAs.,PTM:Phosphorylated. The phosphorylation sites are at the C-terminal part of the protein.,PTM:The N-terminus is blocked.,similarity:Contains 1 HTH La-type RNA-binding domain.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with DDX15. May interact with RUFY1., |
| Subcellular location | Nucleus . |
| Expression | Epithelium,Liver,Placenta,Skeletal muscle, |
Additional Images
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of HeLa cells treated with Forskolin 40nM 30', using SSB (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SSB (Phospho-Ser366) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells, using SSB (Phospho-Ser366) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES7288-100
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