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Antibody Product Overview
| ELK.No | ES7351 |
| Product name | TCF-9 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | GCFC2; C2orf3; GCF; TCF9; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; Transcription factor 9; TCF-9 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 6936 |
| Human Swiss-Prot | P16383 |
| Source | Rabbit |
| Isotype | IgG |
| Target | TCF-9 |
| Fields | |
| Gene name | GCFC2 |
| Protein name | GC-rich sequence DNA-binding factor 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8BKT3 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GCF. AA range:141-190 |
| Specificity | TCF-9 Polyclonal Antibody detects endogenous levels of TCF-9 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 80kD |
| Background | The first mRNA transcript isolated for this gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). A positively charged amino terminus present only in the chimera was determined to bind GC-rich DNA, thus mistakenly thought to identify a transcription factor gene. [provided by RefSeq, Jul 2008], |
| Function | function:Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters.,sequence caution:Contaminating sequence. The N-terminus matches the 2q37.3 region.,similarity:Belongs to the GCF family.,tissue specificity:Widely expressed in tissues and cell lines., |
| Subcellular location | Nucleus, nucleoplasm . Nucleus, nucleolus . |
| Expression | Widely expressed in tissues and cell lines. |
Additional Images
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Western blot analysis of lysates from RAW264.7 cells, using GCF Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using GCF antibody.
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: AO-06-ES7351-50
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