Français
English
Antibody Product Overview
| ELK.No | ES7398 |
| Product name | Thymidine Kinase rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | TK1; Thymidine kinase; cytosolic |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7083 |
| Human Swiss-Prot | P04183 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Thymidine Kinase |
| Fields | >>Pyrimidine metabolism;>>Drug metabolism - other enzymes;>>Metabolic pathways;>>Nucleotide metabolism |
| Gene name | TK1 |
| Protein name | Thymidine kinase cytosolic |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 21877 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P04184 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24834 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P27158 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TK. AA range:1-50 |
| Specificity | Thymidine Kinase Polyclonal Antibody detects endogenous levels of Thymidine Kinase protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 25kD |
| Background | catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family., |
| Function | catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family., |
| Subcellular location | Cytoplasm. |
| Expression | Epithelium,Lymph,Skin,Uterus, |
Additional Images
No image
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
No image
Immunofluorescence analysis of HepG2 cells, using TK Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TK Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Western blot analysis of lysates from COLO205 cells, using TK Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES7398-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package