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Antibody Product Overview
| ELK.No | ES7444 |
| Product name | TPH1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | TPH1; TPH; TPRH; TRPH; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 7166 |
| Human Swiss-Prot | P17752 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Tryptophan Hydroxylase |
| Fields | >>Tryptophan metabolism;>>Folate biosynthesis;>>Metabolic pathways;>>Serotonergic synapse |
| Gene name | TPH1 |
| Protein name | Tryptophan 5-hydroxylase 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 21990 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P17532 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24848 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P09810 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Tryptophan Hydroxylase. AA range:231-280 |
| Specificity | TPH1 Polyclonal Antibody detects endogenous levels of TPH1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 51kD |
| Background | This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009], |
| Function | catalytic activity:L-tryptophan + tetrahydrobiopterin + O(2) = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.,cofactor:Fe(2+) ion.,pathway:Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.,similarity:Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.,similarity:Contains 1 ACT domain.,subunit:Multimer of identical subunits.,tissue specificity:Isoform 2 seems to be less widely expressed than isoform 1., |
| Subcellular location | cytosol,neuron projection, |
| Expression | [Isoform 2]: Seems to be less widely expressed than isoform 1. |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,TPH1 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
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Western Blot analysis of HepG2 cells using TPH1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunofluorescence analysis of HUVEC cells, using Tryptophan Hydroxylase Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Tryptophan Hydroxylase Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES7444-100
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