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Antibody Product Overview
| ELK.No | ES7453 |
| Product name | Tsg 101 rabbit pAb |
| Reactivity | Human;Mouse;Rat;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | TSG101; Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7251 |
| Human Swiss-Prot | Q99816 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Tsg 101 |
| Fields | >>Viral life cycle - HIV-1;>>Endocytosis |
| Gene name | TSG101 |
| Protein name | Tumor susceptibility gene 101 protein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 22088 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61187 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 292925 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q6IRE4 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TSG101. AA range:281-330 |
| Specificity | Tsg 101 Polyclonal Antibody detects endogenous levels of Tsg 101 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 44kD |
| Background | The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008], |
| Function | alternative products:Additional isoforms seem to exist. Several shorter isoforms are detected in primary breast cancers and other tumors,domain:The coiled coil domain may interact with stathmin.,domain:The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.,domain:The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.,function:Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a |
| Subcellular location | Cytoplasm . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Late endosome membrane ; Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Midbody, Midbody ring . Nucleus . Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Nuclear localization is cell cycle-dependent. Interaction with CEP55 is required for localization to the midbody during cytokinesis. . |
| Expression | Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas. |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,Tsg 101 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
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Western blot analysis of lysates from COS7 cells, using TS101 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200
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: AO-06-ES7453-50
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