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Vimentin (phospho Ser83) rabbit pAb
AO-06-ES7506-100
Antibody Product Overview
| ELK.No | ES7506 |
| Product name | Vimentin (phospho Ser83) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | VIM; Vimentin |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 7431 |
| Human Swiss-Prot | P08670 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Vimentin |
| Fields | >>Epstein-Barr virus infection;>>MicroRNAs in cancer |
| Gene name | VIM |
| Protein name | Vimentin |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 22352 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P20152 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 81818 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P31000 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser83. AA range:56-105 |
| Specificity | Phospho-Vimentin (S83) Polyclonal Antibody detects endogenous levels of Vimentin protein only when phosphorylated at S83. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 54kD |
| Background | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009], |
| Function | function:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.,online information:Vimentin entry,PTM:One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.,sequence caution:Intron retention.,similarity:Belongs to the intermediate filament family.,subunit:Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM.,tissue specificity:Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines., |
| Subcellular location | Cytoplasm . Cytoplasm, cytoskeleton . Nucleus matrix . Cell membrane . |
| Expression | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
Additional Images
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Western Blot analysis of 293 cells using Phospho-Vimentin (S83) Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vimentin (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells treated with paclitaxel 1uM 24h, using Vimentin (Phospho-Ser83) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES7506-100
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