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Ku-80 (phospho Thr714) rabbit pAb
AO-06-ES7520-50
Antibody Product Overview
| ELK.No | ES7520 |
| Product name | Ku-80 (phospho Thr714) rabbit pAb |
| Reactivity | Human;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | XRCC5; G22P2; X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair pr |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7520 |
| Human Swiss-Prot | P13010 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Ku-80 |
| Fields | >>Non-homologous end-joining |
| Gene name | XRCC5 |
| Protein name | X-ray repair cross-complementing protein 5 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P27641 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Ku80 around the phosphorylation site of Thr714. AA range:683-732 |
| Specificity | Phospho-Ku-80 (T714) Polyclonal Antibody detects endogenous levels of Ku-80 protein only when phosphorylated at T714. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 83kD |
| Background | The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008], |
| Function | developmental stage:Expression increases during promyelocyte differentiation.,disease:Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86.,domain:The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.,function:Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of t |
| Subcellular location | Nucleus . Nucleus, nucleolus . Chromosome . |
| Expression | Cervix carcinoma,Coronary artery,Heart,Neuroblastoma,Osteoblast,Thy |
Additional Images
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Immunofluorescence analysis of HeLa cells, using Ku80 (Phospho-Thr714) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Ku80 (Phospho-Thr714) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from COS7 cells, using Ku80 (Phospho-Thr714) Antibody. The lane on the right is blocked with the phospho peptide.
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