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Splicing factor 1 (phospho Ser82) rabbit pAb
AO-06-ES7536-50
Antibody Product Overview
| ELK.No | ES7536 |
| Product name | Splicing factor 1 (phospho Ser82) rabbit pAb |
| Reactivity | Human;Mouse;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | SF1; ZFM1; ZNF162; Splicing factor 1; Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7536 |
| Human Swiss-Prot | Q15637 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Splicing factor 1 |
| Fields | |
| Gene name | SF1 |
| Protein name | Splicing factor 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q64213 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SF1 around the phosphorylation site of Ser82. AA range:48-97 |
| Specificity | Phospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 68kD |
| Background | This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016], |
| Function | alternative products:Additional isoforms seem to exist,function:Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.,PTM:Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.,similarity:Belongs to the BBP/SF1 family.,similarity:Contains 1 CCHC-type zinc finger.,similarity:Contains 1 KH domain.,subunit:Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15.,tissue specificity:Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart., |
| Subcellular location | Nucleus. |
| Expression | Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart. |
Additional Images
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Western blot analysis of SH-SY5Y 293T using p-Splicing factor 1 (S82) antibody. Antibody was diluted at 1:500
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Immunofluorescence analysis of A549 cells, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HUVEC cells treated with anisomycin 25ug/ml 30', using SF1 (Phospho-Ser82) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES7536-50
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