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Antibody Product Overview
| ELK.No | ES7560 |
| Product name | Frizzled-5 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | FZD5; C2orf31; Frizzled-5; Fz-5; hFz5; FzE5 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7855 |
| Human Swiss-Prot | Q13467 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Frizzled-5 |
| Fields | >>mTOR signaling pathway;>>Wnt signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Melanogenesis;>>Cushing syndrome;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Basal cell carcinoma;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
| Gene name | FZD5 |
| Protein name | Frizzled-5 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 14367 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9EQD0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 317674 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q8CHL0 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FZD5. AA range:461-510 |
| Specificity | Frizzled-5 Polyclonal Antibody detects endogenous levels of Frizzled-5 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 65kD |
| Background | frizzled class receptor 5(FZD5) Homo sapiens Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq, Jul 2008], |
| Function | domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,domain:The PDZ-binding motif mediates interaction with GOPC.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellul |
| Subcellular location | Cell membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cell junction, synapse . Perikaryon . Cell projection, dendrite . Cell projection, axon . Localized at the plasma membrane and also found at the Golgi. . |
| Expression | Oesophageal carcinoma,Retina, |
Additional Images
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Immunofluorescence analysis of A549 cells, using FZD5 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO cells, using FZD5 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES7560-50
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