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Antibody Product Overview
| ELK.No | ES7566 |
| Product name | CARD 14 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | IHC;IF;ELISA |
| Other name | CARD14; CARMA2; Caspase recruitment domain-containing protein 14; CARD-containing MAGUK protein 2; Carma 2 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 79092 |
| Human Swiss-Prot | Q9BXL6 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CARD 14 |
| Fields | >>NF-kappa B signaling pathway |
| Gene name | CARD14 |
| Protein name | Caspase recruitment domain-containing protein 14 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 170720 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q99KF0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CAR14. AA range:291-340 |
| Specificity | CARD 14 Polyclonal Antibody detects endogenous levels of CARD 14 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 110kD |
| Background | This gene encodes a caspase recruitment domain-containing protein that is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. Members of this protein family are scaffold proteins that are involved in a diverse array of cellular processes including cellular adhesion, signal transduction and cell polarity control. This protein has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012], |
| Function | caution:Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.,function:Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.,similarity:Contains 1 CARD domain.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,subunit:CARD14 and BCL10 bind to each other by CARD-CARD interaction.,tissue specificity:Expressed in placenta. Also detected in HeLa S3 cells, but not in the other cancer cell lines tested., |
| Subcellular location | [Isoform 1]: Cytoplasm .; [Isoform 2]: Cytoplasm .; [Isoform 3]: Cytoplasm . |
| Expression | Isoform 1 is detected in placenta and epidermal keratinocytes (PubMed:22521418). Isoform 2 is detected in leukocytes and fetal brain (PubMed:22521418). |
Additional Images
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Immunofluorescence analysis of MCF7 cells, using CAR14 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES7566-100
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