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Antibody Product Overview
| ELK.No | ES7621 |
| Product name | Ndfip1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | NDFIP1; N4WBP5; PSEC0192; PSEC0223; NEDD4 family-interacting protein 1; Breast cancer-associated protein SGA-1M; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NF-kappa-B-activating protein 164; Putative |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 80762 |
| Human Swiss-Prot | Q9BT67 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Ndfip1 |
| Fields | |
| Gene name | NDFIP1 |
| Protein name | NEDD4 family-interacting protein 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 65113 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8R0W6 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 291609 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q5U2S1 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from Ndfip1 . at AA range: 150-230 |
| Specificity | Ndfip1 Polyclonal Antibody detects endogenous levels of Ndfip1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 25kD |
| Background | The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008], |
| Function | developmental stage:Expressed in fetal brain, kidney and lung.,domain:The WW-binding motifs mediate interaction with NEDD4.,function:May play a role in Golgi structure maintenance.,PTM:Ubiquitinated by NEDD4; mono-, di- and polyubiquitinated forms are detected. Ubiquitination regulates its degradation.,subunit:Interacts with NEDD4. Interacts in vitro with NEDD4L, SR140, WWP2 and ITCH.,tissue specificity:Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta., |
| Subcellular location | Endosome membrane ; Multi-pass membrane protein . Golgi apparatus membrane . Cell junction, synapse, synaptosome . Cell projection, dendrite . Secreted . Detected in exosomes and secreted via the exosomal pathway (PubMed:18819914). |
| Expression | Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung. |
Additional Images
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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES7621-50
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