Français
English
Antibody Product Overview
| ELK.No | ES7754 |
| Product name | NIFK rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | IHC;IF;WB;ELISA |
| Other name | MKI67IP; NIFK; NOPP34; MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 84365 |
| Human Swiss-Prot | Q9BYG3 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NIFK |
| Fields | |
| Gene name | MKI67IP |
| Protein name | MKI67 FHA domain-interacting nucleolar phosphoprotein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 67949 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q91VE6 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NIFK. AA range:200-249 |
| Specificity | NIFK Polyclonal Antibody detects endogenous levels of NIFK protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 34kD |
| Background | This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009], |
| Function | PTM:Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Localizes to mitotic chromosomes in conjunction with MKI67.,subunit:Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis., |
| Subcellular location | Nucleus, nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67. |
| Expression | Brain,Cervix carcinoma,Epithelium,Lung,Plac |
Additional Images
No image
Immunofluorescence analysis of HUVEC cells, using NIFK Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NIFK Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
No image
: AO-06-ES7754-50
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package