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Antibody Product Overview
| ELK.No | ES7789 |
| Product name | Atg4C rabbit pAb |
| Reactivity | Human;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | ATG4C; APG4C; AUTL1; AUTL3; Cysteine protease ATG4C; AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 84938 |
| Human Swiss-Prot | Q96DT6 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Atg4C |
| Fields | >>Autophagy - other;>>Autophagy - animal |
| Gene name | ATG4C |
| Protein name | Cysteine protease ATG4C |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q811C2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ATG4C. AA range:21-70 |
| Specificity | Atg4C Polyclonal Antibody detects endogenous levels of Atg4C protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 49kD |
| Background | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008], |
| Function | enzyme regulation:Inhibited by N-ethylmaleimide.,function:Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.,similarity:Belongs to the peptidase C54 family.,tissue specificity:Highly expressed in skeletal muscle, heart, liver and testis., |
| Subcellular location | Cytoplasm . |
| Expression | Brain,Heart,Placenta,Testis, |
Additional Images
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Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunofluorescence analysis of HUVEC cells, using ATG4C Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ATG4C Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from K562 and COS7 cells, using ATG4C Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES7789-50
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