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Per2 (phospho Ser662) rabbit pAb
AO-06-ES7896-50
Antibody Product Overview
| ELK.No | ES7896 |
| Product name | Per2 (phospho Ser662) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | PER2; KIAA0347; Period circadian protein homolog 2; hPER2; Circadian clock protein PERIOD 2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8864 |
| Human Swiss-Prot | O15055 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Per2 |
| Fields | >>Circadian rhythm;>>Circadian entrainment;>>Transcriptional misregulation in cancer;>>Acute myeloid leukemia |
| Gene name | PER2 |
| Protein name | Period circadian protein homolog 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18627 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O54943 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Period Circadian Protein 2 around the phosphorylation site of Ser662. AA range:636-685 |
| Specificity | Phospho-Per2 (S662) Polyclonal Antibody detects endogenous levels of Per2 protein only when phosphorylated at S662. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 120kD |
| Background | This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014], |
| Function | disease:Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.,function:Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation.,induction:Serum-induced levels in fibroblasts show circadian oscillations. Maximum levels after 1 hour stimulation, minimum levels after 12 hours. Another peak is then observed after 24 hours.,PTM:Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradatio |
| Subcellular location | [Isoform 1]: Nucleus . Cytoplasm . Cytoplasm, perinuclear region . Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Translocate to the nucleus after phosphorylation by CSNK1D or CSNK1E. Also translocated to the nucleus by CRY1 or CRY2. PML regulates its nuclear localization. .; [Isoform 2]: Nucleus, nucleolus . |
| Expression | Widely expressed. Found in heart, brain, placenta, lung, liver, skeleatal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low levels in lung. Isoform 2 is expressed in keratinocytes (at protein level). |
Additional Images
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Western Blot analysis of 3T3 cells using Phospho-Per2 (S662) Polyclonal Antibody diluted at 1:500
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Period Circadian Protein 2 (Phospho-Ser662) Antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using Period Circadian Protein 2 (Phospho-Ser662) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES7896-50
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