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Antibody Product Overview
| ELK.No | ES7915 |
| Product name | Cyclin D3 rabbit pAb |
| Reactivity | Human;Mouse;Rat;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | CCND3; G1/S-specific cyclin-D3 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 896 |
| Human Swiss-Prot | P30281 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Cyclin D3 |
| Fields | >>Cell cycle;>>p53 signaling pathway;>>PI3K-Akt signaling pathway;>>Cellular senescence;>>Wnt signaling pathway;>>Hippo signaling pathway;>>Focal adhesion;>>JAK-STAT signaling pathway;>>Measles;>>Influenza A;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Chemical carcinogenesis - receptor activation |
| Gene name | CCND3 |
| Protein name | G1/S-specific cyclin-D3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12445 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P30282 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25193 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P48961 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cyclin D3. AA range:243-292 |
| Specificity | Cyclin D3 Polyclonal Antibody detects endogenous levels of Cyclin D3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 32kD |
| Background | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008], |
| Function | function:Essential for the control of the cell cycle at the G1/S (start) transition. Potentiates the transcriptional activity of ATF5.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin D subfamily.,subunit:Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K., |
| Subcellular location | Nucleus . Cytoplasm . |
| Expression | Brain,Lung,Placenta,Synovial membrane tissue,Umbilical cord blood, |
Additional Images
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Western Blot analysis of COS7 cells using Cyclin D3 Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human tonsil. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Cyclin D3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COS7 cells, treated with Forskolin 40nM 30', using Cyclin D3 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES7915-50
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