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CD28 (phospho Tyr218) rabbit pAb
AO-06-ES8032-50
Antibody Product Overview
| ELK.No | ES8032 |
| Product name | CD28 (phospho Tyr218) rabbit pAb |
| Reactivity | Human |
| Applications | WB;ELISA |
| Other name | CD28; T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 940 |
| Human Swiss-Prot | P10747 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CD28 |
| Fields | >>Cell adhesion molecules;>>T cell receptor signaling pathway;>>Intestinal immune network for IgA production;>>Type I diabetes mellitus;>>Measles;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Autoimmune thyroid disease;>>Systemic lupus erythematosus;>>Rheumatoid arthritis;>>Allograft rejection;>>Graft-versus-host disease;>>Viral myocarditis |
| Gene name | CD28 |
| Protein name | T-cell-specific surface glycoprotein CD28 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12487 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P31041 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218. AA range:171-220 |
| Specificity | Phospho-CD28 (Y218) Polyclonal Antibody detects endogenous levels of CD28 protein only when phosphorylated at Y218. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 45kD |
| Background | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011], |
| Function | function:Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.,online information:CD28 entry,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70.,tissue specificity:Expressed in T-cells and plasma cells, but not in less mature B-cells., |
| Subcellular location | Membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell surface . |
| Expression | Expressed in T-cells and plasma cells, but not in less mature B-cells. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD28 (Phospho-Tyr218) Antibody
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Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using CD28 (Phospho-Tyr218) Antibody. The lane on the right is blocked with the phospho peptide.
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: AO-06-ES8032-50
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