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Antibody Product Overview
| ELK.No | ES8037 |
| Product name | ZO-2 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | TJP2; X104; ZO2; Tight junction protein ZO-2; Tight junction protein 2; Zona occludens protein 2; Zonula occludens protein 2 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 9414 |
| Human Swiss-Prot | Q9UDY2 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ZO-2 |
| Fields | >>Tight junction;>>Vibrio cholerae infection |
| Gene name | TJP2 |
| Protein name | Tight junction protein ZO-2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 21873 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9Z0U1 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ZO-2. AA range:1063-1112 |
| Specificity | ZO-2 Polyclonal Antibody detects endogenous levels of ZO-2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 160kD |
| Background | This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011], |
| Function | disease:Defects in TJP2 are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.,function:Plays a role in tight junctions and adherens junctions.,similarity:Belongs to the MAGUK family.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 3 PDZ (DHR) domains.,subcellular location:Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures.,subunit:Homodimer, and heterodimer with ZO1. Interacts with occludin, SAFB and UBN1. Interaction with SAFB occurs in the nucleus.,tissue specificity:This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain whereas isoform C1 is expressed at high level in the kidney, pancreas, heart |
| Subcellular location | Cell junction, adherens junction. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction . Nucleus . Also nuclear under environmental stress conditions and in migratory endothelial cells and subconfluent epithelial cell cultures. . |
| Expression | This protein is found in epithelial cell junctions. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues. |
Additional Images
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Western blot analysis of lysate from K562 cells, using ZO-2 antibody.
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: AO-06-ES8037-100
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