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Antibody Product Overview
| ELK.No | ES8522 |
| Product name | SPOP rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC |
| Other name | Speckle-type POZ protein (HIB homolog 1) (Roadkill homolog 1) |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8405 |
| Human Swiss-Prot | O43791 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SPOP |
| Fields | >>Hedgehog signaling pathway |
| Gene name | SPOP |
| Protein name | SPOP |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 20747 |
| Mouse gene link | |
| Mouse Swiss-Prot | Q6ZWS8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human SPOP. AA range:41-90 |
| Specificity | SPOP Polyclonal Antibody detects endogenous levels of SPOP |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 42kD |
| Background | This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008], |
| Function | domain:The MATH domain interacts with H2AFY and BMI1.,function:Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3.,miscellaneous:Antigen recognized by serum from scleroderma patient.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the Tdpoz family.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 1 MATH domain.,subunit:Homodimer. Part of a complex consisting of BMI1, CUL3 and SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Part of a complex consisting of DAXX, CUL3 and SPOP. Interacts with H2AFY, IPF1/PDX1, BMI1 and DAXX. Interacts with CUL3.,tissue specificity:Widely expressed., |
| Subcellular location | Nucleus . Nucleus speckle . |
| Expression | Widely expressed. |
Additional Images
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Western Blot analysis of 3T3 HEPG2 Hela cells using SPOP Polyclonal Antibody diluted at 1:1500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200
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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
: AO-06-ES8522-50
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