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Antibody Product Overview
| ELK.No | ES8612 |
| Product name | RPA14 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC |
| Other name | Replication protein A 14 kDa subunit (RP-A p14) (Replication factor A protein 3) (RF-A protein 3) |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 6119 |
| Human Swiss-Prot | P35244 |
| Source | Rabbit |
| Isotype | IgG |
| Target | RPA14 |
| Fields | >>DNA replication;>>Nucleotide excision repair;>>Mismatch repair;>>Homologous recombination;>>Fanconi anemia pathway |
| Gene name | RPA3 REPA3 RPA14 |
| Protein name | Replication protein A 14 kDa subunit (RP-A p14) (Replication factor A protein 3) (RF-A protein 3) |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 68240 |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9CQ71 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthetic peptide from human protein at AA range: 30-80 |
| Specificity | The antibody detects endogenous RPA14 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 14kD |
| Background | function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RPA4., |
| Function | function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RPA4., |
| Subcellular location | Nucleus . |
| Expression | Brain,Kidney,Lung, |
Additional Images
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Western blot analysis of lysate, antibody was diluted at 2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES8612-100
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