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Antibody Product Overview
| ELK.No | ES8613 |
| Product name | GADD34 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog) |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 23645 |
| Human Swiss-Prot | O75807 |
| Source | Rabbit |
| Isotype | IgG |
| Target | GADD34 |
| Fields | >>Protein processing in endoplasmic reticulum |
| Gene name | PPP1R15A GADD34 |
| Protein name | Protein phosphatase 1 regulatory subunit 15A (Growth arrest and DNA damage-inducible protein GADD34) (Myeloid differentiation primary response protein MyD116 homolog) |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17872 |
| Mouse gene link | |
| Mouse Swiss-Prot | P17564 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthetic peptide from human protein at AA range: 430-490 |
| Specificity | The antibody detects endogenous GADD34 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000, ELISA 1:10000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 73kD |
| Background | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008], |
| Function | function:Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.,induction:By methyl methanesulfonate and ionizing irradiation. By IL24 in melanoma cells; which induces apoptosis.,miscellaneous:The phosphatase activity of the PPP1R15A-PP1 complex toward EIF2S1 is specifically inhibited by Salubrinal, a drug that protects cells from endoplasmic reticulum stress.,PTM:Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.,similarity:Belongs to the PPP1R15 family.,subunit:Interacts with PCNA (By similarity) |
| Subcellular location | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side . Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side . Associates with membranes via an N-terminal amphipathic intramembrane region. . |
| Expression | Brain, |
Additional Images
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Western blot analysis of MOUSE-BRAIN lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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: AO-06-ES8613-100
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