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Rb (Acetyl-K873/K874) rabbit pAb
AO-06-ES8829-100
Antibody Product Overview
| ELK.No | ES8829 |
| Product name | Rb (Acetyl-K873/K874) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC |
| Other name | Retinoblastoma-associated protein (p105-Rb) (pRb) (Rb) (pp110) |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5925 |
| Human Swiss-Prot | P06400 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rb |
| Fields | >>Endocrine resistance;>>Cell cycle;>>Cellular senescence;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Chemical carcinogenesis - receptor activation;>>Pancreatic cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
| Gene name | RB1 |
| Protein name | Rb |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P13405 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized Acetyl peptide derived from human Rb. at AA range: K873/872 |
| Specificity | This antibody detects endogenous levels of Rb at Human:K873+K874;Mouse:K866+K867;Rat:K865+K866, It doesn't reacte with total protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | |
| Background | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008], |
| Function | disease:Defects in RB1 are a cause of bladder cancer [MIM:109800].,disease:Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500].,disease:Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ("cat eye") is investigated.,function:Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts |
| Subcellular location | Nucleus . During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization. . |
| Expression | Expressed in the retina. Expressed in foreskin keratinocytes (at protein level) (PubMed:20940255). |
Additional Images
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Western Blot analysis of 1,mouse-heart 3,mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES8829-100
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