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Antibody Product Overview
| ELK.No | ES8981 |
| Product name | SPHK1 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8877 |
| Human Swiss-Prot | Q9NYA1 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SPHK1 |
| Fields | >>Sphingolipid metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Tuberculosis |
| Gene name | SPHK1 SPHK SPK |
| Protein name | Sphingosine kinase 1 (SK 1) (SPK 1) (EC 2.7.1.91) |
| Human gene link | |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8CI15 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | Q91V26 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 160-240 |
| Specificity | SPHK1 Polyclonal Antibody detects endogenous levels of protein. |
| Formulation | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000 ELISA 1:5000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 42kD |
| Background | The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011], |
| Function | catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.,similarity:Contains 1 DAGKc domain.,subunit:Interacts with ACY1 (By similarity). Binds to calmodulin. Interacts with SPHKAP.,tissue specificity:Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle., |
| Subcellular location | Cytoplasm . Nucleus . Cell membrane . Endosome membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse . Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner (PubMed:19854831). Binds to membranes containing negatively charged lipids but not neutral lipids (PubMed:24929359). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity). . |
| Expression | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level) (PubMed:29662056). |
Additional Images
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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES8981-50
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