Recombinant Anti-Cyclin B1 antibody (Rabbit mAb)

Protein full name

G2/mitotic-specific cyclin-B1

Synonyms

CCNB, CCNB1, Cyclin B1, G2/mitotic specific cyclin B1, Ccn-2, Ccnb1-rs13, Cycb, Cycb1, Ccnb1, CCNB

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1

Uniprot ID

P24860

Isotype

IgG

Clone Number

SB719

Purity

Affinity purification

Predicted MW./Observed MW.

48 kDa / 55 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

HT-29, C6, NIH/3T3

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Images

WB analysis of Cyclin B1(GB150224).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.